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Received: February 20, 2026

Revised: March 2, 2026

Published: March 30, 2026

 

The study was aimed at evaluating the bactericidal activity of the chlorine-containing disinfectant «Blanidas» against representatives of the genus Mycobacterium. The relevance of the research is обусловлена the high environmental resistance of mycobacteria and their significant epizootic and epidemiological importance. The effectiveness of 0,2%, 0,3%, and 1,0% working solutions was assessed at different exposure times and levels of initial microbial contamination using cultures of M. phlei and M. bovis, allowing evaluation of the disinfectant’s effect on both saprophytic and pathogenic species.

It was established that the bactericidal activity of the disinfectant depends on the concentration of the working solution, exposure time, and microbial population density. At a concentration of 0,2%, short-term exposure did not ensure complete inhibition of M. phlei growth; however, prolonged contact resulted in complete inactivation of the culture. Increasing the concentration to 0,3% led to a reduction in microbial contamination after 5 hours of exposure. Application of a 1,0% solution provided a complete bactericidal effect regardless of the initial population density and exposure duration.

Under conditions simulating organic load, wood as a porous material demonstrated higher resistance to the disinfectant compared to ceramic tile, while calico showed intermediate decontamination results. The 1,0% concentration ensured complete inactivation of M. bovis on all tested surfaces, even under high contamination levels and in the presence of organic matter.

The biological assay on laboratory animals confirmed the absence of infection development, further indicating complete pathogen inactivation and the epizootic safety of the applied disinfection regimens. The obtained results are of practical importance for veterinary medicine, as they provide a scientific basis for optimizing the use of chlorine-containing disinfectants in livestock facilities, minimizing pathogen persistence in the environment, and improving biosecurity within the framework of the One Health concept.

 

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