Marker-assisted selection for genotyping herds of hybrid gilts based on population-genetic variability

DOI: 10.32900/2312-8402-2022-127-32-42

Budakva Y. O.,
Ph.D. student,,
Pochernyaev K. F.,
Doctor of Agricultural Sciences, Senior Researcher,,
Institute of Pig Breeding and Agro-Industrial Production of the NAAS,
Korinnyi S. M.,
Candidate of Agricultural Sciences, Senior Researcher,,
Poltava State Agrarian University, Ukraine,
Saienko A. M.,
Candidate of Agricultural Sciences, Senior Researcher,,
Balatsky V. M.,
Doctor of Agricultural Sciences,,
Institute of Pig Breeding and Agro-Industrial Production of the NAAS
Povod M. G.,
Doctor of Agricultural Sciences,,
Sumy National Agrarian University

Keywords: hybrid gilts, (Large White × Landrace) × Maxgro, native, immunologically castrated, SNP, MC4R (c.1426 A>G), RYR1 (g.1843 C>T), CTSD (g.70 G>A), PCR-RFLP analysis, ADG, AGE100


In the study, the allelic effect of single nucleotide polymorphisms of SNPs was determined to estimate fattening productivity in a herd of hybrid gilts (n=101) (Large White × Landrace) × Maxgro. The studied herd of hybrid gilts was obtained as a result of direct (Large White × Landrace) and reciprocal crossing (Landrace × Large White) with boars of the Maxgro terminal line. We conducted a population analysis of polymorphisms by melanocortin genes 4 MC4R (c.1426 A>G), cathepsin D CTSD (g.70 G>A), and ryanodine receptor 1 RYR1 (g.1843 C>T) using software GenAlEX6. To genotyping a herd of hybrid gilts, 2 experimental groups were selected: control (uncastrated) in quantity (n=56) and experimental (immunologically castrated) (n=45) grown in conditions of LLC SPE «Globinsky Pig Farm». Laboratory tests are carried out in the laboratory of genetics in the Institute of Pig Breeding and Agro-Industrial Production of the NAAS of Ukraine. Due to the fact that information about polymorphism on the above SNPs in the population uncastrated and immunologically castrated herd of hybrid pigs which are breed in Ukraine is absent, this requires breeding work involving marker-associated selection on the inside of the breed base, to find out which alleles will be determined as desirable in the marker selection of hybrid pigs. Marker breeding is an effective tool in improving fattening qualities for the pig industry – modern commercial lines of pigs. Therefore, we decided to carry out the typing of an experimental herd of hybrid gilts. The results of genotyping will provide useful information for the selection of the commodity population of hybrid pigs for fixing the desired useful signs of the genotype.  Polymorphism of the SNPs studied MC4R (c.1426 A>G), CTSD (g.70 G>A), and RYR1 (g.1843 C>T) is determined by the method of PCR-RFLP analysis. Thus, a breed character was established distribution of frequencies of encounter ability of alleles of investigated SNPs.


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