Fatty acid composition of total lipids and sorption capacity of bee thorax tissue at different amounts of sunflower oil in feed additive

DOI: 10.32900/2312-8402-2019-122-180-189

Saranchuk Ivan,
Ph.D., Senior Researcher,
Bukovyna State Agricultural Experimental Station of NAAS

Keywords: honey bees, feed supplement, fatty acids, queen bees reproductive ability, bees (workers) honey productivity


In the article, the results of experimental trials are presented on connection investigation between fatty acid composition and sorption capacity of honey bees thorax tissues and productive traits at different amount of sunflower oil in feed additive. It is established, that as a result of adding 10 and 20 g of sunflower oil to a feed consisting of defatted natural soy beans flour and sugar syrup, the content of saturated, monounsaturated, and particularly polyunsaturated fatty acids in the feed additive increases dose-dependently – in fatty acid composition of total lipids, as well as in composition of unesterified fatty acids. Feeding on the additive enriched with sunflower oil causes dose-dependent concentration increase of saturated, monounsaturated, and polyunsaturated fatty acids of total lipids in honey bees thorax tissues in І- and particularly ІІ experimental group. Herewith, in thorax tissue of the above-mentioned bees, the correlation of family ω-3 polyunsaturated fatty acids to family ω-6 polyunsaturated fatty acids decreases. Concentration increase of family ω-3 polyunsaturated fatty acids and particularly of ω-6 ones causes the dose-dependent increase of sorption capacity of honey bee thorax tissues in І and especially ІІ experimental groups. Due to this, in honey bees thorax tissues in the experimental group ІІ, there is an increase of ferum, zinc, cuprum, chromium, plumbum, and cadmium concentration. Changes in fatty acids composition and sorption capacity of honey bee thorax tissues in І and especially ІІ experimental groups are accompanied by changes in queen bees reproductive capacity and working bees honey productivity. Particularly, the queen bees oviposition in І and especially ІІ experimental groups increases by 11,9 and 26,4 %, respectively; and the working bees honey productivity increases by 16,9 and 26,6 %, respectively.


  1. Arien, Y., Dag, A., Zarchin, S., Masci, T., & Shafir, S. (2015). Omega-3 deficiency impairs honey bee learning. Proc. Natl. Acad. Sci. USA, 112(51), 15761–15766.
  2. Couture, P., & Hulbert, A. J. (1995). Membrane fatty acid composition is related to body mass in mammals. The Journal of Membrane Biology, 148(1), 27–39.
  3. Ma, L., Wang, Y., Hang, X., Wang, H., Yang, W., & Xu, B. (2015). Nutritional effect of alpha-linolenic acid on honey bee colony development (Apis mellifera L.). Journal of Apicultural Science, 59(2), 63–72.
  4. Arien, Y., Dag, A., & Shafir, S. (2018). Omega-6:3 Ratio More Than Absolute Lipid Level in Diet Affects Associative Learning in Honey Bees. Front. Psychol., 9, 1–8. doi.org/10.3389/fpsyg.2018.01001.
  5. Gätschenberger, H., Azzami, K., Tautz, J., & Beier, H. (2013). Antibacterial Immune Competence of Honey Bees (Apis mellifera) Is Adapted to Different Life Stages and Environmental Risks. PLoS ONE, 8(6). doi: 10.1371/journal.pone.0066415.
  6. Wu, Y., Zheng, H., Corona, M., Pirk, C., Meng, F., Zheng, Y., & Hu, F. (2017). Comparative transcriptome analysis on the synthesis pathway of honey bee (Apis mellifera) mandibular gland secretions. Scientific Reports, 7(1), 4530. doi: 10.1038/s41598-017-04879-z.
  7. Rabiee, F., Modaresi, M., & Gheisari, A. (2015). The effect to various oleic acid levels on reproductive parameters in queen bee. Der Pharmacia Lettre, 7(12), 326–331.
  8. Hulbert, A. J., Kelly, M. A., & Abbott, S. K. (2014). Polyunsaturated fats, membrane lipids and animal longevity. Journal of Comparative Physiology B: biochemical, systemic, and environmental physiology, 184(2), 149–166.
  9. Hulbert, A. J., & Abbott, S. K. (2011). Nutritional ecology of essential fatty acids: an evolutionary perspective. Australian Journal of Zoology, 59(6), 369–379.
  10. Hulbert, A. J. (2010). Metabolism and longevity: Is there a role for membrane fatty acids? Integrative and Comparative Biology, 50(5), 808–817.
  11. AL-Kahtani, S. N. (2017). Fatty Acids and B Vitamins Contents in Honey Bee Collected Pollen in Relation to Botanical Origin. Scientific Journal of King Faisal University (Basic and Applied Sciences), 18(2), 41–48.
  12. Lavrekhin, F. A., & Pankova, S. V. (1983). Biologiya medonosnoy pchely. 3-ye izd., pererab. i dop. [Biology of the honeybee. 3rd edition, revised and enlarged]. Moscow : Kolos [in Russian].
  13. Rivis, J. F., Shelevach, A. V., Fedak, V. V., Gopanenko, O. О., & Saranchuk, I. I. (2017). Kilkisni khromatohrafichni metody vyznachennya okremykh lipidiv i zhyrnykh kyslot u biolohichnomu materiali: metod. posibnyk [Quantitative chromatographic methods for determination of individual lipids and fatty acids in biological material: method. manual]. Lviv : Spolom [in Ukrainian].
  14. Khavezov, I., & Tsalev, D. (1983). Atomno-absorbtsionnyy analiz [Atomic absorption analysis]. Leningrad : Khimiya [in Russian].
  15. Yakovlev, M. V. (1958). Izucheniye sorbtsionnykh svoystv nekotorykh organov pri eksperimental’nom tuberkuleze metodom okrashivaniya. Issledovaniye svoystv selezenki i legkikh. Soobshcheniye III [The study of the sorption properties of certain organs in experimental tuberculosis by staining. The study of the properties of the spleen and lungs. Message ІІІ]. Byulleten’ eksperimental’noy biologii i meditsiny. – Bulletin of experimental biology and medicine. Moscow: Medgiz, ХІV, 45–54 [in Russian].