Evaluation of oxidative stress biomarkers levels in the equine blood after in vitro treatment with sansevieria caulescens n.e.br. extract

Download PDF

DOI: 10.32900/2312-8402-2019-122-30-42

Tkachenko H.,
Doctor of Biological Sciences,
Osadowski Z.,
Doctor of Biological Sciences,
Institute of Biology and Environmental Protection,
Pomeranian University in Słupsk, Poland,
Buyun L.,
Doctor of Biological Sciences,
Maryniuk M.,
Post-graduatestudent,
Kharchenko I.,
Ph.D.,
M. Gryshko National Botanic Garden, National Academy of Science of Ukraine

Keywords: Sansevieriacaulescens N.E.Br., horses, erythrocytes, 2-thiobarbituric acid reactive substances (TBARS), aldehydic and ketonic derivatives of oxidatively modified proteins, total antioxidant capacity

Abstract

The main goal of present study was to evaluate the level of the 2-thiobarbituric acid reactive substances (TBARS) as lipid peroxidation biomarker, aldehydic and ketonic derivatives of oxidatively modified proteins, and total antioxidant capacity in the equine erythrocytes’ suspension induced by treatment of leaf extracts obtained from Sansevieriacaulescens N.E.Br. There were no significant changes for TBARS level between the value in the control group and in the equine erythrocytes’ suspension after incubation with extracts derived from leaves of S.caulescens(35.88±3.02 μmol/L vs. 35.04±2.31 μmol/L). The level of aldehydic and ketonic derivatives of oxidatively modified proteins was non-significantly changed in the equine erythrocytes’ suspension incubated with an extract obtained from the leaves of S. caulescens(31.16±1.89 nmol/mL vs. 29.77±1.17 nmol/mL for aldehydic derivatives, 39.47±2.20 nmol/mL vs. 36.75±1.73 nmol/ mL for ketonic derivatives of oxidatively modified proteins). The antioxidative and prooxidative mechanism of various Sansevieria species in equine erythrocyte suspension will be further studied in detail. Our study suggests that there were no significant changes for TBARS level as biomarker of lipid peroxidation, aldehydic and ketonic derivatives of oxidatively modified proteins, and total antioxidant capacity between values in control group and in the muscle tissue of rainbow trout after incubation with extracts from leaves of S. caulescens. Taking into account existing experimental evidence, it is reasonable to assume that secondary plant metabolites, i.e. polyphenolic compounds in the extract of S. caulescens may contribute to the antioxidant activity. In conclusion, the antioxidative and prooxidative mechanism of various Sansevieria species in equine erythrocyte suspension will be further studied in detail. The obtained information may be useful in the clinical usage of plants in medicine and veterinary. Finally, these findings justify the traditional uses of Sansevieriaplants for therapeutic purposes.

References

  1. Ahamad, T., Negi, D. S., Khan, F. (2017). Phytochemical analysis, total phenolic content, antioxidant and antidiabetic activity of Sansevieria cylindricaleaves extract. Journal of Natural Products and Resources, 3(2), 134-136. DOI:10.21767/2472-0151.100026.
  2. Ajila, M., Naidu, K. A., Bhat, U. J. S., Rao, P. (2007). Bioactive compounds and antioxidant potential of mango peel extract. Food Chem., 105, 982-988. DOI: 10.1016/j.foodchem.2007.04.052.
  3. Alagawany, M., Farag, M. , El-Kholy, M. S., El-Sayed, S. A. A., Dhama,K. (2016). Effect of resveratrol, cinnamaldehyde and their combinations on the antioxidant defense system and ATP release of rabbit erythrocytes: in vitro study. Asian J. Anim. Sci. Vet. Adv., 12, 1-9. DOI: 10.3923/ajava.2017.1.9.
  4. Andhare, R. , Raut, M. K., Naik, S. R. (2012). Evaluation of antiallergic and anti-anaphylactic activity of ethanolic extract of Sanseveiriatrifasciata leaves(EEST) in rodents. J. Ethnopharmacol., 142(3), 627-633. DOI: 10.1016/ j.jep.2012.05.007.
  5. Bhattacharjee, N., Khanra, R., Dua, T. , Das, S., De, B., Zia-Ul-Haq, M., De Feo, V., Dewanjee, S. (2016). SansevieriaroxburghianaSchult. & Schult. F. (Family: Asparagaceae) Attenuates Type 2 Diabetes and Its Associated Cardiomyopathy. PLoS One, 11(11), e0167131.DOI: 10.1371/journal.pone.0167131.
  6. Buyun,, Maryniuk, M., Tkachenko, H., Osadowski, Z. (2017). Antibacterial evaluation of an ethanolic extract from SansevieriatrifasciataPrain against Staphylococcus aureus. Proceedings of the International Scientific and Practical Internet Conference “Problems and perspectives of modern agricultural science“. Mykolaiv : Mykolaiv SDDS IAZ, p. 88.
  7. Buyun,, Tkachenko, H., Osadowski, Z., Maryniuk, M. (2016). Antibacterial activity of certain Sansevieriaspecies against Staphylococcus aureus. Słupskie PraceBiologiczne, 13, 19-36.
  8. Chhabra, S. C., Mahunnah, R. A., Mshiu, E. N. (1987). Plants used in traditional medicine in Eastern Tanzania. I. Pteridophytes and Angiosperms (Acanthaceae to Canellaceae). Journal of Ethnopharmacology, 21, 253-277. DOI:10.1016/0378-8741(87)90103-6.
  9. Dubinina, E. , Burmistrov, S. O., Khodov, D. A., Porotov, I. G. (1995). Oxidative modification of human serum proteins. A method of determining it. Vopr. Med. Khim., 41, 24-26 [In Russian].
  10. Farag, M. , Alagawany, M. (2018). Erythrocytes as a biological model for screening of xenobiotics toxicity. Chem. Biol. Interact., 279, 73-83. DOI: 10.1016/ j.cbi.2017.11.007.
  11. Galaktionova, L. P., Molchanov, A. V., Elchaninova, S. A., Varshavskiy, B. Y. (1998). The lipid peroxidation processes in patients with ulcerous illness of stomach and duodenum. Lab. Diagnostics, 6, 10-14 [In Russian].
  12. Haldar, P. , Kar, B., Bala, A., Bhattacharya, S., Mazumder, U. K. (2010). Antitumor activity of Sansevieriaroxburghiana rhizome against Ehrlich ascitescarcinoma in mice. Pharm. Biol., 48(12), 1337-1343. DOI: 10.3109/ 13880201003792592.
  13. Kahkonen, M. P., Hopia, A. I., Vuorela, H. , Raucha, J. P., Pihlaja, K., Kujala, T. S. (1999). Antioxidant activity of plant extracts containing phenolic compounds. J. Agric. Food Chem., 47, 3954-3962. DOI: 10.1021/jf990146l.
  14. Kamyshnikov, V. (2004). Reference book on clinic and biochemical researches and laboratory diagnostics,MEDpress-inform, Moscow [In Russian].
  15. Karamova, N., Gumerova, S., Hassan, G. O., Abdul-Hafeez, E. , Ibrahim,O. H. M., Orabi, M. A. A., Ilinskaya, O. (2016). Antioxidant and antimutagenic potential of extracts of some Agavaceae family plants. BioNanoScience, 6(4), 591-593. DOI: 10.1007/s12668-016-0286-x.
  16. Khalumba, M. , Mbugua, P. K., Kung’u, J. B. (2005). Uses and conservation of some highland species of the genus Sansevieria Thunb. in Kenya. African Crop Science Conference Proceedings, 7, 527-532.
  17. Kiringe, J. (2006). A survey of traditional health remedies used by the Maasai of Southern Kaijiado District, Kenya. Ethnobotany Research and Applications, 4, 61-73.
  18. Levine, R. L., Garland, D., Oliver, C. N., Amici, A., Climent, I., Lenz, A.-G., Ahn, B.-W., Shaltiel, S., Stadtman, E. (1990). Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol., 186, 465-478. DOI: 10.1016/0076-6879(90)86141-h.
  19. Lykkesfeldt, J. (2007). Malondialdehyde as biomarker of oxidative damage to lipids caused by smoking. Chim. Acta, 380,50-58. DOI:10.1016/j.cca.2007.01.028.
  20. Maheshwari, R., Shreedhara, C. , Polu, P. R., Managuli, R. S., Xavier,S. K., Lobo, R., Setty, M., Mutalik, S. (2017). Characterization of the Phenolic Compound, Gallic Acid from SansevieriaroxburghianaSchult and Schult. f. Rhizomes and Antioxidant and Cytotoxic Activities Evaluation. Pharmacogn. Mag., 13 (Suppl 3), S693-S699. DOI: 10.4103/pm.pm_497_16.
  21. Maryniuk, M., Tkachenko, H., Buyun, L., Kharchenko, I., Osadowski, Z. (2018). Oxidative stress biomarkers in the muscle tissue of the rainbow trout (Oncorhynchus mykissWalbaum) after in vitro treatment of SansevieriacaulescensE.Br. extract. Agrobiodiversity for Improving Nutrition, Health and Life Quality, (2), 111-123. DOI: 10.15414/agrobiodiversity.2018.2585-8246.111-123.
  22. PackiaLincy, M., Mohan, V. , Jeeva, S. (2014). In vitro evaluation of antioxidant activity of aerial part of Maeruaapetala. Roth (Jacobs) (Capparaceae). Asian J. Pharm. Clin. Res., 7(2), 77-81.
  23. Pagano, M., Faggio, C. (2015). The use of erythrocyte fragility to assess xenobiotic cytotoxicity. Cell Biochem. Funct., 33(6), 351-355. DOI: 10.1002/cbf.3135.
  24. Staples, G. , Herbst, D. R. (2005). A Tropical Garden Flora: Plants cultivated in the Hawaiian Island and other tropical places. Bishop Museum Press, Honolulu, Hawaii.
  25. Takawira-Nyenya, T., Newton, L.E., Wabuyele, E., Stedje, B. (2014). Ethnobotanical uses of Sansevieria (Asparagaceae) in Coast Province of Kenya. Ethnobotany Research and Application, 12(1), 51-69.
  26. Tkachenko, H., Buyun,, Osadowski, Z., Maryniuk, M. (2017). The antibacterial screening of certain Sansevieria species against Escherichia coli strain. Youth and Progress of Biology: Book of Abstracts of XIIІ International Scientific Conference for Students and Ph.D. Students (Lviv, 25–27 April 2017). Lviv, p. 220–221.
  27. Tkachenko, H., Buyun,, Osadowski, Z., Maryniuk, M. (2017). The antibacterial activity of certain SansevieriaThunb. species against Escherichia coli. Agrobiodiversity for improving nutrition, health, and life quality, 1, 446-453. DOI:10.15414/agrobiodiversity.2017.2585-8246.446-453.
  28. Tkachenko, H., Buyun,, Pażontka-Lipiński, P., Witaszek, M., Osadowski,Z. (2017). In vitro protective effect of extracts obtained from various Sansevieria species against oxidative damage of proteins in equine erythrocytes. SłupskiePraceBiologiczne, 14, 247-265.
  29. Wang, H., Cao, G., Prior, R. (1997). Oxygen radical absorbing capacity of anthocyanins. J. Agric. Food Chem., 45(2), 304-309. DOI: 10.1021/jf960421t.
  30. Watt, J. , Breyer-Brandwijk, M. G. (1962). The Medicinal and Poisonous Plants of Southern and Eastern Africa. E & S Livingstone Ltd., Edinburgh, Scotland.
  31. Wolfe, K., Xianzhong, W. , Liu, R. H. (2003). Antioxidant activity of apple peels. J. Agric. Food Chem., 51, 609-614. DOI: 10.1021/jf020782a.
  32. Zar, J. (1999). Biostatistical Analysis. 4th ed., Prentice-Hall Inc., Englewood Cliffs, New Jersey.