Keywords: rapeseed oil, olive oil, rice oil, rosemary essential oil, 2-thiobarbituric acid reactive substances, lipid peroxidation
We hypothesized that rosemary essential oil would inhibit or reduce lipid oxidation in plant oils due to the antioxidative properties. The aim of this study was to investigate the content of 2-thiobarbituric acid reactive substances (TBARS) in the various plant oils (rapeseed oil, olive oil, rice oil) with the use of rosemary essential oil (Etja, Elblag, Poland) as antioxidant agent by monitoring the lipid peroxidation during one month storage period.
The effect of the interaction of the addition of REO and storage time on TBARS value in the rapeseed oil.
Lipid oxidation increased significantly (p<0.001) during storage, particularly in the control sample (the rapeseed oil), which showed the highest increase at 8 days (19-fold increased) and at 40 days (14.4-fold increased). The REO decreased lipid oxidation compared to the control sample by 23.9 % (p<0.05) at 8 days and by 9.4 % (p>0.05) at 40 days. At 15 days, the TBARS values reached approximately 22.31 μmols∙mL-1, corresponding to a lipid oxidation increase of approximately 33.8% (p>0.05) for samples enriched by REO.
The rosemary essential oil decreased lipid oxidation in the rapeseed oil compared to the control sample by 23.9 % (p<0.05) at 8 days and by 9.4 % (p>0.05) at 40 days. Addition of rosemary essential oil to olive oil increased significantly TBARS values only at 8 days of the storage. The reduction of the lipid oxidation was the highest at 40 days as compared to the start of the study. Rosemary essential oil added to rice oil induced the increase of TBARS level at 8 days (by 23.7 %, p<0.05) and 0 days (by 64.4 %, p<0.05), respectively. Lipid peroxidation retarding capacity of rosemary essential oil was found obviously promising. These antioxidant activities seem to be attributed to antioxidant compounds present in the rosemary essential oil.
Consequently, rosemary essential oil could be successfully added to various plant oils as a natural antioxidant source for feeding animals. The knowledge of extent and mode of inhibition of specific compounds, which are present in plant essential oils, may contribute to the successful application of such natural preservatives in foods since certain combinations of second metabolites provide a high antioxidant activity.
- al-Sereiti, M. R., & Abu-Amer, K. M., Sen, P. (1999). Pharmacology of rosemary (Rosmarinus officinalis Linn.) and its therapeutic potentials. Indian J. Exp. Biol. 37(2),124–130.
- Begum, A., & Sandhya, S., Shaffath Ali, S., Vinod, K. R., Reddy, S., Banji, D. (2013). An in-depth review of the medicinal flora Rosmarinus officinalis (Lamiaceae). Acta Sci. Pol. Technol. Aliment., 12(1), 61–73.
- Bolumar, T., & LaPeña, D., Skibsted, L. H., Orlien, V. (2016). Rosemary and oxygen scavenger in active packaging for prevention of high-pressure induced lipid oxidation in pork patties. Food Packaging and Shelf Life, 7, 26–33.
- Bozin, B., & Mimica-Dukic, N., Samojlik, I., Jovin, E. (2007). Antimicrobial and antioxidant properties of rosemary and sage (Rosmarinus officinalis L. and Salvia officinalis L., Lamiaceae) essential oils. J. Agric. Food Chem., 55(19), 7879–7885.
- de Oliveira, J. R., & Camargo, S.E.A., de Oliveira, L. D. (2019). Rosmarinus officinalis L. (rosemary) as therapeutic and prophylactic agent. J. Biomed. Sci., 26(1), 5.
- European Medicines Agency. Community herbal monograph on Rosmarinus officinalis L., aetheroleum. 2010. [http://www.ema.europa.eu/docs/en_GB/docu-ment_library/Herbal_Community_herbal_monograph/2011/01/WC500101493.pdf]
- Kadri, A., & Zarai, Z., Ben Chobba, I., Bekir, A., Gharsallah, N., Damak, M., Gdoura, R. (2011). Chemical constituents and antioxidant properties of Rosmarinus officinalis L. essential oil cultivated from South-Western Tunisia. J. Med. Plants Res., 5, 5999–6004.
- Kamyshnikov, V. S. (2004). A reference book on the clinic and biochemical researches and laboratory diagnostics, MEDpress-inform, Moscow.
- Karoui, R., & Hassoun, A. (2017). The efficiency of Rosemary and Basil Essential Oils on the Shelf-Life Extension of Atlantic Mackerel (Scomberscombrus) Fillets Stored at 2°C. J. AOAC Int., 100(2), 335–344.
- Kumar, Y., & Yadav, D. N., Ahmad, T., Narsaiah, K. (2015). Recent Trends in the Use of Natural Antioxidants for Meat and Meat Products. Comprehensive Reviews in Food Science and Food Safety, 14, 796–812.
- Martin, D., & Salas-Perez, L., Villalva, M., Vázquez L., Garcia-Risco, M.R., Jaime, L., Reglero, G. (2017). Effect of alkylglycerol-rich oil and rosemary extract on oxidative stability and antioxidant properties of a cooked meat product. European Journal of Lipid Science and Technology, 118, 1600412.
- Napoli, E. M., & Curcuruto, G., Ruberto, G. (2010). Screening of the essential oil composition of wild Sicilian Rosemary. Biochemical Systematics and Ecology, 38, 659–670.
- Ojeda-Sana, A. M., & van Baren, C. M., Elechosa, M. A., Juarez, M. A., Moreno, S. (2013). New insights into antibacterial and antioxidant activities of rosemary essential oils and their main components. Food Control, 31, 189–195.
- Patel, S. (2015). Plant essential oils and allied volatile fractions as multifunctional additives in meat and fish-based food products: a review. Food Addit. Contam. Part A Chem. Anal. Control Expo Risk Assess., 32(7), 1049–1064.
- Prabuseenivasan, S, & Jayakumar, M, Ignacimuthu, S. (2006). In vitro antibacterial activity of some plant essential oils. BMC Complement.Altern. Med., 6, 39.
- Qiu, X., & Jacobsen, C., Sørensen, A. M. (2018). The effect of rosemary (Rosmarinus officinalis L.) extract on the oxidative stability of lipids in cow and soy milk enriched with fish oil. Food Chem., 263, 119–126.
- Rašković, A., & Milanović, I., Pavlović, N., Ćebović, T., Vukmirović, S., Mikov, M. (2014). Antioxidant activity of rosemary (Rosmarinus officinalis L.) essential oil and its hepatoprotective potential. BMC Complement. Altern. Med., 14, 225.
- Realini, C. E., & Marcos, B. (2014). Active and intelligent packaging systems for a modern society. Meat Science, 98, 404–419.
- Saoudi, S., & Chammem, N., Sifaoui, I., Bouassida-Beji, M., Jiménez, I. A., Bazzocchi, I. L., Silva, S. D., Hamdi, M., Bronze, M. R. (2016). Influence of Tunisian aromatic plants on the prevention of oxidation in soybean oil under heating and frying conditions. Food Chem., 212, 503–511.
- Sirocchi, V., & Devlieghere, F., Peelman, N., Sagratini, G., Maggi, F., Vittori, S., Ragaert, P. (2017). Effect of Rosmarinus officinalis L. essential oil combined with different packaging conditions to extend the shelf life of refrigerated beef meat. Food Chem., 221, 1069–1076.
- Teixeira, B., & Marques, A., Ramos, C., Neng, N. R., Nogueira, J.M.F., Saraiva, J. A., Nunes, M. L. (2013). Chemical composition and antibacterial and antioxidant properties of commercial essential oils. Ind.Crop Prod., 43, 587–595.
- Vicente, G., & Molina, S., González-Vallinas, M., García-Risco, M. R., Fornari, T., Reglero, G., de Molina, A. R. (2013). Supercritical rosemary extracts, their antioxidant activity, and effect on hepatic tumor progression. The Journal of Supercritical Fluids, 79, 101–108.
- Vital, A. C., & Guerrero, A., MonteschioJde, O., Valero, M. V., Carvalho, C. B., de Abreu Filho, B.A., Madrona, G. S., do Prado, I.N. (2016). Effect of Edible and Active Coating (with Rosemary and Oregano Essential Oils) on Beef Characteristics and Consumer Acceptability. PLoS One, 11(8), e0160535.
- Xie, J., & VanAlstyne, P., Yang, X. (2017). A review on rosemary as a natural antioxidation solution. European Journal of Lipid Science and Technology, 119, 1600439.
- Zar, J. H. (1999). Biostatistical Analysis, 4th ed., Prentice Hall Inc., New Jersey.