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The chronology of scientific research achievements in the process of determining the biological nomenclature and classification of honey bees and the implementation of the results obtained for use in the practice of beekeeping in the selection and preservation of aboriginal breeds (races) of bees in modern environmental conditions is highlighted. An attempt is made to focus this study in the field of analysis of the chronology of methods used in the historical aspect of determining the taxonomy of insects, in particular bees. At the same time, the problems and prospects of scientific research in modern economic and natural and climatic conditions of the development of the industry are reflected. It is shown that initially only morphometry was used worldwide to identify bee breeds. However, morphometric features are not always informative in identifying subspecies, since they are subject to variability under the influence of environmental conditions. Later, biochemical methods for identifying bee subspecies based on polymorphism of allozyme loci were developed. It is shown that at the same time, methods for identifying bee subspecies based on polymorphism of mitochondrial DNA (mtDNA) loci were developed. This polymorphism was successfully used in phylogenetic and phylogeographic studies of honey bees. The disadvantage of mtDNA markers is the exclusively maternal type of inheritance. At the same time, methods for identifying bee subspecies were developed taking into account polymorphism of nuclear DNA (nDNA) loci. Recently, methods for identifying bee subspecies based on SNP analysis have been developed. These markers have become widely used in population, evolutionary and phylogenetic studies of bees due to the development of next-generation sequencing methods NGS (Next Generation Sequencing) Illumina. SNP markers are characterized by high resolution due to their number and stable inheritance over several generations, which can be successfully used in genetic mapping, population and evolutionary studies, selection of lines for economically useful traits and disease resistance, identification of taxonomic affiliation of bee families.

Referenses

Alburaki, M., Bertrand, B., Legout, H., Moulin, S., Alburaki, A., Sheppard, W. S. and Garnery, L. (2013). A fifth major genetic group among honeybees revealed in Syria‘, BMC Genetics, 14. doi: 10.1186/1471-2156-14-117.

Babu K. N., Sheeja Th. E., Minoo D.,  Rajesh M. K., Samsudeen K, Suraby E.J., Payatatti I., Kumar V.  (2020). Random Amplified Polymorphic DNA (RAPD) and Derived Techniques Molecular Plant Taxonomy. Methods and Protocols DOI: 10.1007/978-1-0716-0997-2_13

Bouga M, Alaux C, Bienkowska M, Bü chler R, Carreck NL, Cauia E, Chlebo R, Dahle B, Dall’Olio R, De la Rú a P, Gregorc A, Ivanova E, Kence A, Kence M, Kezic N, Kiprijanovska H, Kozmus P, Kryger P, Le Conte Y, Lodesani M, Murilhas AM, Siceanu A, Soland G, Uzunov G& Wilde J. (2011). A review of methods for discrimination of honey bee populations as applied to European beekeeping. J. Apicult. Res. 50(1): 51–84. https://doi.org/10.3896/IBRA.1.50.1.06).

Brodschneider, R., Gray, A., Van der Zee, R, Adjlane, N., Brusbardis, V., Charrière, J.D., Chlebo R.,Coffey, M.F., Crailsheim, K., Dahle, B., anihlík, J., Danneels, E., De Graaf, D.C., Draži M.M., Fedoriak, M., Forsythe, I., Golubovski, M., Gregorc, A., Grzda, U., Hubbuck, I., Tunca, R.., Kauko, L., Kilpinen, O., Kretavicius, J., Kristiansen, P., Martikkala, M., Martín-Hernández, R., Mutinelli, F., Peterson, M., Otten, C., Ozkirim, A., Raudmets, A., Simon-Delso, N., Soroker, V., Topolska, G., Vallon, J., Vejsnæs, F., and Woehl, S. (2016). Preliminary anlysis of loss rates of honey bee colonies during winter 2015/16 from the COLOSS survey, J. Apicult. Res. vol. 55, no. 5, pp. 375–8. doi: 10.1080/00218839.2016.1260240

Chen C., Liu Z., Pan Q., Chen X., Wang H., Guo H. (2016). Genomic analyses reveal demographic history and temperate adaptation of the newly discovered honey bee subspecies Apis mellifera sinisxinyuan n. ssp. // Molecular Biology and  Evolution..  33: 1337-1348. doi: 10.1093/molbev/msw017

Cherevatov O.V., Panchuk I.I., Kerek S.S., Volkov R.A. (2019). Molekulyarne rIznomanIttya speysernoYi dIlyanki SOI-COII mItohondrialnogo genomu ta pohodzhennya karpatskoYi bdzholi / O.V.Cherevatov, I.I.Panchuk, S.S.Kerek, R.A. Volkov // TsitologIya ta genetika., T. 53. № 4. – S.13–19

Cherevatov V.F., Babenko V.V., Galatyuk O.E., Yarovets V.I. (2023). Klasichna morfologIya kril bdzholi medonosnoYi (Apis mellifera L.) z vikoristannyam protokolu DAWINO / V.F.Cherevatov, V.V.Babenko, O.E.Galatyuk, V.I. Yarovets // BIologIchnI sistemi. T. 15. Vip. 2. 179–187

Cherkasova A. Y. Davidenko Y. K. Huba P. A. (1991). Slovar-spravochnyk po pchelovodstvu. K. 416 s.

Crozier RH, Crozier YC. (1992). The cytochrome b and ATPase genes of honeybee mitochondrial DNA. Mol Biol Evol. May; 9 (3):474-482. doi: 10.1093/oxfordjournals.molbev.a040729.

Diniz-Filho H.R, Radloff S, Fuchs S. (2000). Spatial analysis of morphometrical variation in African honeybees (Apis mellifera L.) on a continental scale. Apidologie. 31(2): 191–204.

Engel, M.S. (1999). The taxonomy of recent and fossil honeybees (Hymenoptera: Apidae; Apis). Journal of Hymenoptera Research 8(2): 165–196. BHL

Franck, P., Garnery, L., Solignac, M. and Cornuet, J. (2000). Molecular confirmation of a fourth lineage in honeybees from the Near East‘, Apidologie, 31,: 167–180. doi:10.1051/apido:2000114.

Franck P, Garnery L, Loiseau A, Oldroyd BP, Hepburn HR, Solignac M, Cornuet JM, (2001). Genetic diversity of the honeybee in Africa: microsatellite and mitochondrial data, Heredity, 86, 420-30.

Garnery L., Solignac M., Selebrano G., Cornuet J., (1993). A simple test using restricted PCR-amplified mitochondrial-DNA to study the genetic-structure of Apis mellifera L. Experientia 49(11): 1016–1021. doi.org/10.1007/BF02125651

Hall A., Slack T., Smith G., Whitson D. (1991). Sport in Canadian Society. Toronto: McClelland & Stewart,. Pp. 281.

Huzenko E. V., Tsar A. Y., Lemesh V. A. (2022). Metody identyfikatsii taksonomichnoi prynalezhnosti medonosnykh bdzhil Apis Mellifera. Molekuliarna i prykladna henetyka. Tom 32,  doi.org/10.47612/1999-9127-2022-32-107-120

Ilyasov R.A., Poskryakov A.V., Nikolenko A.G. (2016). Organizatsiya genoma medonosnoy pchelyi. Biomika, Tom 8,  2,  97–99

Ilyasov R.A., Dar S.A., Dukku U.H.3, Kandemir I., Li M.L., Ozkan Kosa A., Nikolenko A.G., Kvon H.V. (2019). Obzor sovremennoy taksonomii evropeyskiy pchel roda Apis. Biomika, Tom 11 # 2, S. 212-241

Kandemir, Özkan A., Fuchs S. (2011). Reevaluation of honey bee (Apis mellifera) microtaxonomy: a geometric morphometric approach.  Apidologie. 42: 618-627. doi: 10.1007/s13592-011-0063-3

Kotthoff U., Wappler T., Engel M.l S. (2013). Greater past disparity and diversity hints at ancient migrations of European honey bee lineages into Africa and Asia // Journal of Biogeography. V. 40 P. 1832–1838. doi: 10.1111/jbi.12151.

Linnaeus, C. 1758. Systema Naturae per regna tria naturae, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis. Editio Decima, Reformata. Tomus I. Holmiæ (Stockholm): impensis direct. Laurentii Salvii. 824 pp. DOI: 10.5962/bhl.title.542 BHL Reference page.  [first availability, see p. 576]

Maa T.C. (1953). An enquiry into the systematics of the tribus Apidini or honey bees (Hymenoptera). Treubia. 21: 525-640.

Magnus R. M., Amber D. T., Szalansky A. L. (2014). Mitochondrial DNA diversity of Honey Bees (Apis mellifera) from unmanaged colonies and Swarms in the Unated States. R.M. Magnus,. Biochem. Genetic V. 52. 245 – 257.

2Meixner M. D, Pinto M. A., Bouga M., Kryger P., Ivanova E., Fuchs S. (2013). Standard methods for characterising subspecies and ecotypes of Apis mellifera. Journal of Apicultural Research 52(4): DOI 10.3896/IBRA.1.52.4.05

Michener, C. D. (2000). The Bees of the World. 2715 North Charles Street Baltimore, Maryland 21218-4363: The John Hopkins University Press. Available at: www.press.jhu.edu.

Metlitska O.I. PolIschuk V.P., Taran S.I. (2010). Zastovuvannya metodIv morfometrIYi ta molekulyarno-genetichnoYi otsInki pri viznachennI chistoporodnostI ukraYinskih bdzhIl / BIlologIya tvarin (naukovo-teoretichniy zhurnal): zb. nauk. pr.  LvIv: IBT NAAN UkraYini,  12, №1. P. 254-259.

Metlitska O. I., Kopilova K. V., (2012). PolImorfIzm mItohondrialnoYi DNK dlya otsInki genetichnoYi strukturi I pIdvidovoYi klasifIkatsIYi ukraYinskih bdzhIl  Visnik PoltavskoYi derzhavnoYi agrarnoYi akademIYi. № 2 113-117

Metlytska, O. I., Kopylov K. V., Berezovskyi O. V. (2016). Suchasni molekuliarno-henetychni pidkhody dlia pidvyshchennia efektyvnosti selektsiinoho protsesu v tvarynnytstvi Ukrainy. Rozvedennia i henetyka tvaryn. Vyp. 51. S. 193-200. – Available at:  http://nbuv.gov.ua/UJRN/rgt_2016_51_28

Pinto M A., Muñoz I., Chávez-Galarza J., Pinto M A. (2012). The Atlantic side of the Iberian peninsula: a hot-spot of novel African honey bee maternal diversity. Apidologie 43: 663-673. doi.org/10.1007/s13592-012-0141-1

Ruttner F. (1973). Zuchttechnik und Zuchtauslese bei der Biene: Anleitungen zur Aufzucht von Königinnen und zur Kör- und Belegstellenpraxis. Ehrenwirth Verlag; 138 p.

Ruttner F. (1988).Biogeography and taxonomy of honeybees. Springer: Berlin/Heidelberg, Germany; 284 p.

Shaibi T., Lattorff H., Moritz R. (2008). A microsatellite DNA toolkit for studying population structure in Apis mellifera. Mol. Ecol. Resour. V. 8, № 5. P. 1034 – 1036.

Sheppard W. S., Meixner M. D. Apis mellifera pomonella, a new honey bee subspecies from Central Asia Apidologie 34 (2003) 367-375 DOI: 10.1051/apido:2003037

Smith D.R, Glenn T.C. (1995) Allozyme polymorphisms in Spanish honeybees (Apis mellifera iberica). Journal of Heredity. 86(1):12-16

Tofilski A. (2008) Using geometric morphometrics and standard morphometry to discriminate three honeybee subspecies. Apidologie.; 39: 558–563. http://10.1051/apido:2008037).

Van der Zee, R., Pisa L., Andonov, S., Brodschneider, R., Charrière, J.D., Chlebo, R., Coffey, M.F., Crailsheim, K., Dahle, B., Gajda, A., Gray, A., Drazic, M.M., Higes, M., Kauko, L., Kence, A., Kence, M., Kezic, N., Kiprijanovska, H., Kralj, J., Kristiansen, P., Hernandez, R.M., Mutinelli, F., Nguyen, B.K., Otten, C., Özkrm, A., Perna, S.F., Peterson, M., Ramsay, G., Santrac, V., Soroker, V.,Topolska, G., Uzunov, A., Vejsnæs, F., Wei, S., Wilkins, S. (2009). Managed honey bee colony losses in Canada, China, Europe, Israel and Turkey, for the winters of 2008–9 and 2009–10, J. Apicult. Res., vol. 51, no. 1, pp. 100–14. doi:10.3896/IBRA.1.51.1.12.

Wallberg, A., Han, F., Wellhagen, G., Dahle, B.,( 2014). A worldwide survey of genome sequence variation provides insight into the evolutionary history of the honeybee Apis mellifera, Nat. Genet., , vol. 46, pp. 1081–8. doi: 10.138/ng.3077.

Whitfield, C.W., Behura, S.K., Berlocher, S.H., Clark, A.G., Johnston, J.S., Sheppard, W.S., Smith, D.R., Suarez, A.V., Weaver, D., and Tsutsui, N.D., (2006). Thrice out of Africa: ancient and recent expansions of the honey bee, Apis mellifera, Science, , vol. 314, pp. 642–5. doi: 10.1126/science.1132772.

Yarovets V.I.,Babenko V.V., Galatyuk O.E. (2022). MorfometrIya kril bdzhIl za vIsmoma oznakami (Indeksami): CI, DBI, DISC.SH., PCI, RI, CI.2, CI.2.1, CI.3 doi.org/10.46913/beekeepingjournal..8.10 65-71.

Vinutha R Bhatta, Naresh Kumar A. (2022). Morphometric Characters of Apis cerana indica Worker Bees under Urban, Rural and Wild Habitats. Applied Ecology and Environmental Sciences. 2022; 10(10): 614-621. doi.org/10.12691/aees-10-10-3).

Zlotin O. Z. (1988). Sviiski komakhy [Relative insects]. K.: Radianska shkola (in Ukrainian)