An in vitro assessment of the anti-aeromonas properties of leaf extract obtained from ficus lyrata warb. (moraceae)

DOI: 10.32900/2312-8402-2019-122-18-30

Pękala-Safińska A.,
Doctor of Biological Sciences, D.V.M.,
Department of Fish Diseases, National Veterinary Research Institute, Pulawy, Poland,
Tkachenko H.,
Doctor of Biological Sciences,
Osadowski Z.,
Doctor of Biological Sciences,
Institute of Biology and Environmental Protection, Pomeranian University in Słupsk, Poland,
Buyun L.,
Doctor of Biological Sciences,
M.M.Gryshko National Botanic Garden, National Academy of Science of Ukraine,
Honcharenko V.,
Ivan Franko Lviv National University,
Prokopiv A.,
Botanic Garden of Ivan Franko Lviv National University

Keywords: Ficus lyrata, Ficus lyrata Bambino, Aeromonas sobria, Aeromonas hydrophila, Aeromonas salmonicida subsp. salmonicida, antimicrobial activity, disc diffusion technique, ethanolic extracts


The aim of the present study was to assess the antibacterial efficacy of ethanolic extracts derived from F. lyrata and its cultivar F. lyrata ‘Bambino’ against three Aeromonas strains (Aeromonas sobria, Aeromonas hydrophila, Aeromonas salmonicida subsp. salmonicida). The leaves of plants were collected in M. M. Gryshko National Botanic Garden (NBG, Kyiv, Ukraine) and Botanic Garden of Ivan Franko Lviv National University (Lviv, Ukraine). Freshly collected leaves were weighed and homogenized in 96 % ethanol (in proportion 1:10) at room temperature. Three Aeromonas strains: Aeromonas sobria (K825) and Aeromonas hydrophila (K886), as well as Aeromonas salmonicida subsp. salmonicida (St30), originated from freshwater fish species such as common carp (Cyprinus carpio L.) and rainbow trout (Oncorhynchus mykiss Walbaum), respectively, were isolated in Department of Fish Diseases, The National Veterinary Research Institute in Pulawy (Poland). Bacteria were collected from fish exhibiting clinical disorders. Our results demonstrated that three Aeromonas strains (Aeromonas sobria, Aeromonas hydrophila, Aeromonas salmonicida subsp. salmonicida) were resistant to ethanolic extract derived from F. lyrata. The inhibition zone diameters were (9.50±0.33 mm), (9.38±0.38 mm), and (9.5±0.5 mm) for Aeromonas sobria, Aeromonas hydrophila and Aeromonas salmonicida subsp. salmonicida (St30), respectively. F. lyrata ‘Bambino’ extract exhibited the intermediate activity against Aeromonas sobria (inhibition zone diameter was 12±0.73 mm), while Aeromonas hydrophila and Aeromonas salmonicida subsp. salmonicida (St30) were resistant (inhibition zone diameters were 9.18±0.54 mm and 9.13±0.44 mm). These results pave the way for the possible development of natural additives to replace synthetic ones. Therefore, further investigations for the isolation of active constituents and their pharmacological evaluation as well as in vitro and in vivo study are required.


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