Keywords: Dendrobium Sw., extracts, antioxidant activity, muscle tissue, rainbow trout, Oncorhynchus mykiss Walbaum
This research aimed to evaluate the in vitro effect of buffer extract obtained from leaves and pseudobulbs (modified shoots) of Dendrobium parishii Rchb. f. on the 2-thiobarbituric acid reactive substances (TBARS) as lipid peroxidation biomarker, aldehydic and ketonic derivatives of oxidatively modified proteins, and total antioxidant capacity (TAC) in the muscle tissue of the rainbow trout (Oncorhynchus mykiss Walbaum). The shoots (pseudobulbs) with leaves of Dendrobium parishii cultivated under glasshouse conditions were sampled at M.M. Gryshko National Botanic Garden (NBG) (Kyiv, Ukraine). Since 1999, the whole collection of tropical and subtropical plants (including orchids) has had the status of a National Heritage Collection of Ukraine and is supported through State funding. Besides, NBG’s collection of tropical orchids was registered at the Administrative Organ of CITES in Ukraine (Ministry of Environment Protection, registration No. 6939/19/1-10 of 23 June 2004). The collected pseudobulbs and leaves were brought into the laboratory for biochemical studies. Freshly collected leaves were washed, weighed, crushed, and homogenized in 0.1M phosphate buffer (pH 7.4) (in proportion 1:19, w/w) at room temperature. The extract was then filtered and investigated for its antioxidant capacity. The extract was stored at -20°C until use. The increase in TBARS level in the muscle tissue exposed to extracts derived from leaves and pseudobulbs of D. parishii was insignificant. The level of ketonic derivatives of oxidatively modified proteins was non-significantly decreased both for leaf and pseudobulb extracts compared to the untreated samples. The extracts obtained from leaves and pseudobulbs of D. parishii significantly increased the TAC level in muscle tissue due to inhibited the Fe2+/ascorbate-induced oxidation of Tween 80. Overall, these findings demonstrate that aqueous extracts of vegetative organs of Dendrobium parishii can enhance the total antioxidant capacity in the muscle tissue of the rainbow trout. Moreover, this antioxidant effect was more intensive for pseudobulb extracts.
- Abu, F., Mat Taib, C. N., Mohd Moklas, M. A., & Mohd Akhir, S. (2017). Antioxidant Properties of Crude Extract, Partition Extract, and Fermented Medium of Dendrobium sabin Evid. Based Complement. Alternat. Med., 2907219.
- Alam, M. N., Bristi, N. J., & Rafiquzzaman, M. (2013). Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm. J., 21(2), 143–152.
- Buyun, L., Tkachenko, H., Kurhaluk, N., Gyrenko, O., Kovalska, L., & Osadowski, Z. (2019). Assessment of oxidative stress biomarkers in the equine blood after in vitro incubation with leaf extract obtained from Dendrobium parishiiF. Agrobiodiversity for Improving Nutrition, Health, and Life Quality, 3, 416–427.
- Buyun, L. I., & Grakhov, V. P. (2014). Bioactivity of some orchid seeds substances and secondary metabolites composition. Proceedings of the 2-nd Russian Conference with international participation dedicated to the memory of Professor A. P. Melikiann «Carpology and reproductive biology of higher plants». (1–3 October 2014), (pр. 188–194). Moscow.
- Cheng, J., Dang, P. , Zhao, Z., Yuan, L. C., Zhou, Z. H., Wolf, D., & Luo, Y. B. (2019). An assessment of the Chinese medicinal Dendrobium industry: Supply, demand and sustainability. J. Ethnopharmacol., 229, 81–88.
- Citarasu T. (2010). Herbal biomedicines: a new opportunity for aquaculture industry. Int., 18, 403–414.
- Dubinina, E. E., Burmistrov, S. O., Khodov, D. A., & Porotov, I. G. (1995). Oxidative modification of human serum proteins. A method of determining it. Voprosy Meditsinskoĭ Khimii, 41, 24–26 [in Russian].
- Galaktionova, L. P., Molchanov, A. V., El’chaninova, S. A., & Varshavskiĭ, Bla. (1998). Lipid peroxidation in patients with gastric and duodenal ulcers. Klinicheskaia Labaratornaia Diagnostika, 6, 10–14 [in Russian].
- Galina, J., Yin, G., Ardó, L., & Jeney, (2009). The use of immunostimulating herbs in fish. An overview of research. Fish Physiol. Biochem., 35(4), 669–676.
- Harikrishnan, R., Balasundaram, C., & Heo, M.-S. (2011). Impact of plant products on innate and adaptive immune system of cultured finfish and shellfish. Aquaculture, 317, 1–15.
- Honda, C., & Yamaki, M. (2000). Phenanthrenes from Dendrobium plicatile. Phytochemistry, 53(8), 987–990.
- Ivannikov, R. V., Laguta, I. V., Stanislavskaya, O. N., Anishchenko, V. M., Buyun, L. I., & Pakhlov, E. M. (2019). Composites for prolonged release of bioactive compounds based on orchid leaves extracts and fumed silica. Nac. Akad. Nauk Ukr., 4, 66–73. [in Ukrainian]
- Kamyshnikov, V. S. (2004). A reference book on the clinic and biochemical researches and laboratory diagnostics. MEDpress-inform, Moscow.
- Kasote, D. M., Katyare, S. S., Hegde, M. V., & Bae, H. (2015). Significance of antioxidant potential of plants and its relevance to therapeutic applications. J. Biol. Sci., 11(8), 982–991.
- Lee, C. T., Kuo, H. C., Chen, Y. H., & Tsai, M. Y. (2018). Current Advances in the Biological Activity of Polysaccharides in Dendrobium with Intriguing Therapeutic Potential. Med. Chem., 25(14), 1663–1681.
- Levine, R. L., Garland, D., Oliver, C. N., Amic, A., Climent, , Lenz, A. G., Ahn, B. W., Shaltiel, S., & Stadtman, E. R. (1990). Determination of carbonyl content in oxidatively modified proteins. Methods in Enzymology, 186, 464–478.
- Li, X. L., & Hong, M. (2020). Aqueous extract of Dendrobium officinale confers neuroprotection against hypoxic-ischemic brain damage in neonatal rats. Kaohsiung Med. Sci., 36(1), 43–53.
- Liu, D., Dong, Z., Xiang, F., Liu, H., Wang, Y., Wang, Q., & Rao, J. (2020). Dendrobium Alkaloids Promote Neural Function After Cerebral Ischemia-Reperfusion Injury Through Inhibiting Pyroptosis Induced Neuronal Death in both In Vivo and In Vitro Neurochem. Res., 45(2), 437–454.
- Luo, A., He, X., Zhou, S., Fan, Y., He, T., & Chun, Z. (2009). In vitro antioxidant activities of a water-soluble polysaccharide derived from Dendrobium nobile extracts. Int. J. Biol.Macromol.,45(4), 359–363.
- Luo, Q. L., Tang, Z. H., Zhang, X. F., Zhong, Y. H., Yao, S. Z., Wang, L. S., Lin, C. W., & Luo, X. (2016). Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale. J. Biol. Macromol., 89, 219–227.
- Ng, T. B., Liu, J., Wong, J. H., Ye, X., Wing Sze, S. C., Tong, Y., & Zhang, K. Y. (2012). Review of research on Dendrobium, a prized folk medicine. Microbiol. Biotechnol., 93(5), 1795–1803.
- Nie, J., Tian, Y., Zhang, Y., Lu, Y. L., Li, L. S., & Shi, J. S. (2016). Dendrobium alkaloids prevent Aβ25-35-induced neuronal and synaptic loss via promoting neurotrophic factors expression in mice. Peer J., 4, e2739.
- Nie, X., Chen, Y., Li, W., Lu, Y. (2020). Anti-aging properties of Dendrobium nobile: From molecular mechanisms to potential treatments. J. Ethnopharmacol., 257, 112839.
- Paudel, M. R., Chand, M. B., Pant, B., Pant, B. (2018). Antioxidant and cytotoxic activities of Dendrobium moniliforme extracts and the detection of related compounds by GC-MS. BMC Complement. Altern. Med., 18(1), 134.
- Peng, H., Yang, M., Guo, Q., Su, T., Xiao, Y., & Xia, Z. Y. (2019). Dendrobium officinale polysaccharides regulate age-related lineage commitment between osteogenic and adipogenic differentiation. Cell Prolif., 52(4), e12624.
- Yamashita, Y., Katagiri, T., Pirarat, N., Futami, K., Endo, M., & Maita, M. (2009). The synthetic antioxifant, etoxyquin, adversely affects immunity in tilapia (Oreochromis niloticus). Aquaculture Nutrition, 15, 144–151.
- Zar J. H. (1999). Biostatistical Analysis. 4th, Prentice-Hall Inc., Englewood Cliffs, New Jersey.
- Zhai, X. F., Wang, J., Zheng, S., & Xiao, X. C. (2017). Protective effects of Dendrobium candidum on ischemia reperfusion-induced arrhythmia. Modern Food Science & Technol., 33(7), 1–8.
- Zhang, J. Y., Guo, Y., Si, J. P., Sun, X. B., Sun, G. B., & Liu, J. J. (2017). A polysaccharide of Dendrobium officinale ameliorates H2O2-induced apoptosis in H9c2 cardiomyocytes via PI3K/AKT and MAPK pathways. J. Biol. Macromol., 104(Pt A), 1–10.
- Zhang, X., Luo, Y., Wei, G., Li, Y., Huang, Y., Huang, J., Liu, C., Huang, R., Liu, G., Wei, Z., & Du, S. (2019). Physicochemical and Antioxidant Properties of the Degradations of Polysaccharides from Dendrobium officinale and Their Suitable Molecular Weight Range on Inducing HeLa Cell Apoptosis. Based Complement. Alternat. Med., 2019: 4127360.
- Zhang, X., Zhang, S., Gao, B., Qian, Z., Liu, J., Wu, S., & Si, J. (2019). Identification and quantitative analysis of phenolic glycosides with antioxidant activity in methanolic extract of Dendrobium catenatum flowers and selection of quality control herb-markers. Food Res. Int., 123, 732–745.
- Zhao, X., Dou, M., Zhang, Z., Zhang, D., & Huang, C. (2017). Protective effect of Dendrobium officinale polysaccharides on H2O2-induced injury in H9c2 cardiomyocytes. Pharmacother., 94, 72–78.
- Zheng, W., & Wang, S. Y. (2003). Oxygen radical absorbing capacity of phenolics in blueberries, cranberries, chokeberries, and lingonberries. Agric. Food Chem., 51(2), 502–509.